Catalogue Number: CT0035075
Description: All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.
Synonyms: Human transferrin, Siderophilin.
Molecular Weight: 76-81 kDa.
CAS Number: 11096-37-0
Formulation: 10mg/mL water solution.
Source: Human blood plasma.
Physical Appearance: Sterile filtered clear or pink solution.
Purity: Greater than 97.0% as determined by agarose gel electrophoresis.
Endotoxin: tested
Iron Concentration: See table below
Pack Size: See table below
Storage & Stability: Transferrin in solution should be stored at -20°C. Please avoid freeze-thaw cycles.
Suitability: Cell culture tested.
Others: Non-reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2