CAS 92921-25-0 保存条件 Store at -20℃. 注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 产品介绍 分子式：C15H9O9N2NaS 分子量：416.29 MDL：MFCD00054983 纯度：≥80% 性状：白色粉末。 溶解性：溶于水。 用途：交联剂；生化试剂； 分子结构式： 背景资料 MBS and its water-soluble analog Sulfo-MBS are heterobifunctional crosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these crosslinkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneously with the maleimide (sulfhydryl-targeted) reaction.